منابع مشابه
Chemically-modified graphenes for oxidation of DNA bases: analytical parameters.
We studied the electroanalytical performances of chemically-modified graphenes (CMGs) containing different defect densities and amounts of oxygen-containing groups, namely graphite oxide (GPO), graphene oxide (GO), thermally reduced graphene oxide (TR-GO) and electrochemically reduced graphene oxide (ER-GO) by comparing the sensitivity, selectivity, linearity and repeatability towards the oxida...
متن کاملSelection of DNA aptamers with two modified bases.
The nucleobases comprising DNA and RNA aptamers provide considerably less chemical diversity than protein-based ligands, limiting their versatility. The introduction of novel functional groups at just one of the four bases in modified aptamers has recently led to dramatic improvement in the success rate of identifying nucleic acid ligands to protein targets. Here we explore the benefits of addi...
متن کاملRelease of sulfur mustard-modified DNA bases by Escherichia coli 3-methyladenine DNA glycosylase II.
The toxic effects of sulfur mustard have been attributed to DNA modification with the formation of 7-hydroxyethylthioethyl guanine, 3-hydroxyethylthioethyl adenine and the cross-link, di-(2-guanin-7-yl-ethyl)sulfide. To investigate the action of bacterial 3-methyladenine DNA glycosylase II (Gly II) on these adducts, calf thymus DNA was modified with [14C]sulfur mustard and used as a substrate f...
متن کاملSeparation of up to 1000 bases on a modified A.L.F. DNA sequencer.
A modified version of the standard A.L.F DNA sequencer (Pharmacia, Uppsala) is described that allows the separation of up to 1000 bases per clone. Fragments were resolved at single peak resolution up to 700 bases. The raw data accuracy was >99.7%, resulting in an overall error rate of <0.01% for the final sequence after sequencing both DNA strands. The modifications include 60 cm thermostatic a...
متن کاملOrdered distribution of modified bases in the DNA of a dinoflagellate.
In DNA of the dinoflagellate Crypthecodinium cohnii, 38% of the thymine is replaced by the modified base 5-hydroxymethyluracil, and approximately 3% of the cytosine is replaced by 5-methylcytosine. Both of the modified bases are non-randomly distributed in the DNA. Determinations of 3' nearest neighbors show that HOMeU is preferentially located in the dinucleotides HOMeUpA and HOMeUpC. Pyrimidi...
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ژورنال
عنوان ژورنال: Current Opinion in Chemical Biology
سال: 2020
ISSN: 1367-5931
DOI: 10.1016/j.cbpa.2020.01.014